The top three women's seeds are all out of the French Open after Agnieszka Radwanska lost to Ajla Tomljanovic in the third round.
While not as big a shock as either Serena Williams or Li Na's exits, it was a sizeable upset nonetheless and leaves fourth seed Simona Halep as the highest-ranked player left in the tournament.
Radwanska has never enjoyed playing on clay, particularly in the slow and heavy conditions that have been a feature of the first week at Roland Garros this year.
She reached the quarter-finals for the first time last year but was on the back foot from the start against 21-year-old Croatian Tomljanovic and went down 6-4 6-4.
It was a breakthrough at the top level for the Croatian world number 72, who is through to the fourth round at a grand slam for the first time at her maiden French Open.
This has been a tournament where the young guns have made their mark, and the powerful Tomljanovic joins Garbine Muguruza, Kristina Mladenovic and Taylor Townsend as a headline maker.
Tomljanovic, Muguruza and Mladenovic, the conquerors of the world's top three in Paris, were all born in 1993.
The Croatian said: "After seeing the two first seeds go out, you kind of feel like 'I can do this too'. I grew up with these girls that are beating them.
"I went on the stadium for the first time, and she kind of feels like home there, because she's been there a lot more than I did. I went out there, and I really, inside really thought I could win. I think that showed and it is why I won."
Tomljanovic moved to America eight years ago to train at Chris Evert's academy and remains close with the 18-time grand slam singles winner.
"She really wants me to do well, and I can feel that from her," said the Croatian. "I'm really happy that she's someone I can rely on."
Radwanska pulled the first set back from 5-1 to 5-4 but Tomljanovic held on and then moved ahead early in the second.
"I don't think was my day today," said Radwanska.
"I think I just started too slow. I was 5?1 down, and I guess I was a little late for the first set. I was still trying, I almost came back, but it didn't work out well.
"But she definitely played good tennis today. I had my chances. I didn't take them. I think that cost me the match."
Radwanska did not feel a major opportunity had presented itself following the exit of the top two seeds.
The Pole said: "I think the draw is so big and it's still the third round. I think it doesn't really matter if someone is out or not. Maybe that matters a little bit later in the second week, but it doesn't matter now."